Fstl1 Knockout cell line (NIH/3T3)

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Fstl1 Knockout cell line (NIH/3T3) Order

Fstl1 Knockout cell line (NIH/3T3)
Catalog#
YKO-M269
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Fstl1 Knockout cell line (NIH/3T3)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
Fstl1 Knockout cell line (NIH/3T3)
Cell Morphology
Fibroblast-like, adherent
Passage ratio
1:3~1:5
Species
Gene Name
Fstl1
Gene ID
14314
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 3 (based on transcript Fstl1-201)
Validation: PCR+Sanger Sequencing
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:NIH/3T3		 STR Info (Cell bank)
Cell Line:NIH 3T3
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 17.019.017.019.0
4-2 19.320.319.320.3
6-7 12.012.0
19-2 10.011.012.011.012.0
1-2 13.017.013.017.0
7-1 29.029.0
8-1 15.015.0
1-1 10.010.0
3-2 14.015.014.015.0
2-1 9.09.0
15-3 20.320.3
6-4 16.015.3
13-1 16.216.2
11-2 15.017.015.017.0
17-2 13.014.013.014.0
12-1 20.020.0
5-5 14.015.014.015.0
X-1 25.025.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Fstl1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the NIH/3T3 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Fstl1 gene knockout Fstl1 Knockout cell line (NIH/3T3) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Fstl1 Knockout cell line (NIH/3T3)
Fstl1 Knockout cell line (NIH/3T3)
Catalog#
YKO-M269
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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